Bleomycin-induced strand-scission of DNA. Mechanism of deoxyribose cleavage.
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چکیده
منابع مشابه
Bleomycin and talisomycin sequence-specific strand scission of DNA: a mechanism of double-strand cleavage.
Computer analyses of DNA sequencing data obtained using various restriction fragments of pBR 322 DNA indicate that a trinucleotide sequence (-Pyr-G-C-) is the most preferred site for cleavage by the antitumor antibiotic bleomycin A2. Talisomycin A, a structurally related bleomycin analog, cleaved at the sequences -G-T/A- most preferentially. However, the presence of a pyrimidine at the 5' side ...
متن کاملThe DNA cleavage mechanism of iron-bleomycin. Kinetic resolution of strand scission from base propenal release.
The action of iron-bleomycin and O2 in cleaving DNA has been resolved into two kinetic events following the initial attack on DNA by the kinetically competent drug species, "activated bleomycin." At 4 degrees C, DNA strand scission, monitored both viscometrically and fluorimetrically (t1/2 = 2.5-5 min), precedes the release from DNA of nucleic base propenals, which is half complete in about 40 ...
متن کاملMechanism of double-strand DNA cleavage effected by iron-bleomycin.
We have observed that in the absence of hydrogen peroxide the Fe(III)-bleomycin (BLM) complex exhibits high DNA cleavage efficiency, converting supercoiled Form I DNA (pBR322 or phix174) to Form II (nicked, relaxed circular); the present study may give an important clue to elucidate the fact that iron-bleomycin mediated double-strand DNA cleavage requires at least one molecule of oxygen (O2) ov...
متن کاملBleomycin and Talisomycin Sequence-specific Strand Scission of DMA: A Mechanism of Double-Strand Cleavage1
Computer analyses of DNA sequencing data obtained using various restriction fragments of pBR 322 DNA indicate that a trinucleotide sequence (-Pyr-G-C-) is the most preferred site for cleavage by the antitumor antibiotic bleomycin A2. Taliso mycin A, a structurally related bleomycin analog, cleaved at the sequences -G-T/Amost preferentially. However, the pres ence of a pyrimidine at the 5' side ...
متن کاملSingle-strand scission and repair of DNA in mammalian cells by bleomycin.
DNA single-strand breakage by bleomycin treatment of cultured mammalian cells was demonstrated by the method of alkaline elution. Elution patterns from treated L1210 cells indicated that part of the DNA was extensively broken while the remainder was affected to a lesser degree. This biphasic effect, which was less prominent in human fibroblasts, may reflect a selective sensitivity either of par...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1981
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)68888-5